radA [2017-05-17 11:12:48]

6-O-methylguanine-DNA methyltransferase, negative effector of DisA activity, participates in the stabilization and/or processing of holliday junction intermediates

Locus: BSU00870

Isoelectric point: 8.13

Molecular weight: 49.32 kDa

Protein length: 458 aa

Gene length: 1374 bp

Function: homologous recombination , control of c-di-AMP formation

Product: 6-O-methylguanine-DNA methyltransferase

Essential: no

Synonyms: sms, yacJ

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Metabolism of signalling nucleotides
• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Other proteins
• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
• Lifestyles → Coping with stress → Heat shock proteins

Gene

Coordinates: 106,096 → 107,472

The protein

Protein family

• RadA subfamily (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ctsR-mcsA-mcsB-clpC-radA-disA
  Description: PubMed

  Sigma factors

  • SigM: sigma factor, PubMed, in SigM regulon
  • SigA: sigma factor, PubMed, in SigA regulon
  • SigB: sigma factor, PubMed PubMed, in SigB regulon
  • SigF: sigma factor, PubMed, in SigF regulon

  Regulatory mechanism

  • CtsR: repression, PubMed, in CtsR regulon
  • Spx: activation, PubMed, in Spx regulon

  Regulation

  • expressed during germination and spore outgrowth PubMed
  • induction during diamide stress (Spx) PubMed

Biological materials

Mutant

• MGNA-B931 (yacJ::erm), available at the NBRP B. subtilis, Japan
• BKG1 (radA::spc), available in Jörg Stülke's lab
• BKG2 (radA-disA::spc), available in Jörg Stülke's lab

Expression vector

• pGP2693: expression in E. coli, with C-terminal Strep-tag, in pGP574, available in Jörg Stülke's lab

References

Loading